Alkaline Phosphatase Labeling Kit

Alkaline Phosphatase Labeling Kit

$300.00

BC1002. In stock N/A .

Description

Alkaline Phosphatase Labeling Kit or Antibody-AP Conjugation Kit enables rapid conjugation of alkaline phosphatase (AP) to an antibody or a protein. The kit includes a modified enzyme (A-AP) and an antibody modifier. The typical coupling efficiency of AP to an antibody is greater than 95%, while the significant coupling is observed within one hour. The kit can also conjugate AP to other proteins.

Alkaline phosphatase labeling kit

Advantages

•Modified alkaline phosphatase with high enzymatic activity, ready for conjugation
•Modified enzyme aliquoted in 3 vials for multiple coupling reactions
•A long spacer (47 atoms) between antibody and phosphatase
•High efficiency
•No homo-crosslinking
•Stable and permanent conjugation
•Coupling at a pH range of pH7.0-9.0
•No reducing reagents
•No catalysts
•Easy protocol

Product specification
Name Alkaline Phosphatase Labeling Kit
Alkaline Phosphatase (A-AP) 3 x 100 μgs at 1-5 mg/ml
Antibody modifier One vial
Desalting column 3 columns
Recommended antibody 3 x 1 00 μgs
Coupling group Amines
Compatible pH 7.0-9.0
Compatible buffer HEPES, MES, MOPS
Application In vitro research use only
Delivery and storage
Delivery Cold packs
Short Term Storage see package
Long Term Storage see package
Other Notes n/a

For custom alkaline phosphatase conjugation, please click here to contact us or email info@clickbios.com.

Alkaline phosphatase labeling kit

1. How to prepare the labeling protein?
The labeling protein must have reactive amine (NH2) groups. It should be purified prior to conjugation. The protein should be prepared in an amine-free buffer. Sodium azide and protein additives should also be avoided. In addition, the desired protein concentration is greater than 2 mg/ml.

2. How much protein can be conjugated?
The kit contains 3 x 100 µgs modified alkaline phosphatase that is sufficient to couple 3x 100 µgs antibody for the optimum result. For coupling of other proteins, the reaction may require to optimize. In addition, the modified enzyme is supplied in the solution form that is convenient for multiple coupling reactions.

3. Is the purification of the conjugate required?
No. Although a small amount of unconjugated enzyme may be present, it can be washed away at the assay step. Therefore, it does not interfere with the assay.

4. Does your company offer custom conjugate for alkaline phosphatase?
Yes. We offer custom bioconjugation services for alkaline phosphatase. Please click here to contact us or email info@clickbios.com.