Description

Antibody Binding Firefly Luciferase is a Protein G-Firefly Luciferase (protein G-Fluc) fusion protein. It consists of protein G and firefly luciferase (Fluc), while the two proteins connect via a short peptide. Protein G tightly binds to IgG molecules from rabbit, human, mouse, goat, sheep, cow, and rat. In addition, protein G conjugates are especially valuable for detecting antibodies that bind poorly to protein A, such as human IgG3, mouse IgG1, and immunoglobulins from rat, sheep, and horse. Firefly luciferase (Fluc) is a light-emitting enzyme to estimate gene expression. It catalyzes the oxidation of firefly luciferin, allowing its detection by a luminometer. As an antibody binding luciferase, protein G-Fluc can avoid harmful labeling of the detecting antibody. Because staining protocols vary with application, the appropriate dilution of Antibody Binding Firefly Luciferase should be determined empirically.

Product specification

Name	Protein G-firefly luciferase
Accession No.	n/a
Target Protein Sequence	n/a
Tag Info	His-tagged
Molecular Weight	85.3 kDa
Source	Recombinant fusion protein from E.coli
Amount	100 μg
Concentration	0.5 ~ 1.0 mg/ml
Purity	≥ 90% by SDS-PAGE
Form	Liquid
Buffer	50 mM sodium phosphate,100 mM NaCl, 1 mM DTT, pH7.2, 50% glycerol
Endotoxin level	not determined
Application	In vitro research use only
Other Features	IgG binding Firefly luciferase
Typical Gel Image
Datasheet & COA	Please contact us to get it.

Delivery and storage

How Does Antibody Binding Firefly Luciferase Work?

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