Description

Antibody Binding GFP is a Protein G-GFP fusion protein. It consists of protein G and enhanced green fluorescent protein (EGFP), while the two proteins connect via a short peptide. Protein G tightly binds to IgG molecules from rabbit, human, mouse, goat, sheep, cow, and rat. Since protein A binds poorly to human IgG3, mouse IgG1, and immunoglobulins from rat, sheep, and horse, protein G conjugates are especially valuable for detecting these antibodies. EGFP has an excitation and emission spectra of 488/509nm, allowing its detection under fluorescent microscopy and flow cytometry. As an antibody binding GFP, Protein G-EGFP protein can avoid harmful labeling of the detecting antibody. Because staining protocols vary with application, the appropriate dilution of Antibody Binding GFP should be determined empirically.

Product specification

Name	Protein G-GFP
Accession No.	n/a
Target Protein Sequence	n/a
Tag Info	N-terminal 8x His-tagged
Molecular Weight	51.6 kDa
Source	Recombinant fusion protein from E.coli
Amount	100 μg
Concentration	0.5 ~ 1.0 mg/ml
Purity	≥ 90% by SDS-PAGE
Form	Liquid
Buffer	50 mM sodium phosphate,100 mM NaCl, 1 mM DTT, pH7.2, 50% glycerol
Endotoxin level	not determined
Application	In vitro research use only
Other Features	Binds to IgG
Typical Gel Image
Datasheet & COA	Please contact us to get it.

Delivery and storage

How Does Antibody Binding GFP Work?

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