Horseradish Peroxidase Conjugation Kit

Horseradish Peroxidase Conjugation Kit

$300.00

BC1001. In stock N/A .

Description

Horseradish Peroxidase Conjugation Kit or HRP Conjugation Kit enables rapid conjugation of horseradish peroxidase (HRP) to an antibody or a protein. The HRP Conjugation Kit includes a modified HRP and the antibody modifier. The conjugation efficiency of HRP to the antibody can reach 95%, while significant coupling is observed within one hour. The HRP Conjugation Kit can also conjugate HRP to other proteins.

HRP Labeling Kit

Advantages

•Modified HRP with high enzymatic activity (> 250 U/mg), ready for conjugation
•Modified enzyme aliquoted in 3 vials for multiple coupling reactions
•A long hydrophilic spacer between antibody and HRP
•High efficiency
•No homo-crosslinking
•Stable and permanent conjugation
•Coupling at a pH range of pH7.0-9.0
•No reducing reagents
•No catalysts
•Easy protocol

Product specification
Name HRP Conjugation Kit
Modified HRP (A-HRP) 3 x 100 μgs
Antibody modifier One vial
Desalting column 3 columns
Recommended antibody 3 x 1 00 μgs (up to 3 x 400 μgs)
Coupling group Amines
Compatible pH 7.0-9.0
Compatible buffer PBS, HEPES, MES, MOPS
Application In vitro research use only
Delivery and storage
Delivery Cold packs
Short Term Storage see package
Long Term Storage see package
Other Notes n/a

For custom HRP conjugation, please click here to contact us or email info@clickbios.com.

HRP Labeling Kit

1. How to prepare the labeling antibody?
The labeling protein must have reactive amine (NH2) groups that are provided by lysine residues or the N-terminal amino acid. It should be purified prior to conjugation. The antibody should be prepared in amine-free buffer (PBS, MES, MOPS, HEPES, etc.) at 2.0 mg/ml or higher. Sodium azide and protein additives should be avoided.

2. How much antibody can be conjugated in each reaction?
Each kit contains 3 x 100 µgs modified HRP that is sufficient for 3 coupling reactions of 100 µgs antibodies for the optimum result. However, up to 400 μgs antibodies may be conjugated.

3. Is the purification of the conjugate required?
No. Although a small amount of unconjugated antibody and horseradish peroxidase may be present, in most applications, they do not interfere with the assay.

4. Can I use the HRP Conjugation Kit to conjugate a protein other than an antibody?
Yes. The coupling reaction may require to optimize.

5. Does your company offer custom HRP conjugation?
Yes. We offer custom bioconjugation services for HRP. Please click here to contact us or email info@clickbios.com.