$0.00
HRV3C Protease (GST-tag, His-tag)
$225.00
Description
Product Overview
HRV3C Protease (GST-tag, His-tag) is a recombinant form of human rhinovirus (HRV) type 14 3C protease. The enzyme recognizes a specific sequence LEVLFQ/GP and cleaves between Gln (Q) and Gly (G). Our HRV3C Protease (GST-tag, His-tag) is useful for removal of tag sequences from proteins having the cleavage site. It can also cleave small peptides with the processing site. Moreover, our HRV3C protease has both His-tag and GST-tag, allowing easy removal from the sample after cleavage.
HRV3C Protease (GST-tag, His-tag) has great stability and robust activity at 4°C. In addition, it is active in buffers that are suitable for most proteins. Typically, 1μg of the HRV3C Protease (GST-tag, His-tag) can cleave 100 μg of target protein at 4°C. However, the cleavage efficiency may vary due to the sequences around the cleavage site and conformation of the target protein. More enzyme, longer cleavage time, or higher temperature (37°C) may enhance cleavage efficiency.
Product specification
| Name | HRV3C Protease (His, GST-tag) |
| Accession No. | P03303 |
| Target Protein Sequence | n/a |
| Tag Info | His-tag, GST-tag |
| Molecular Weight | 47.4 kDa |
| Source | Recombinant fusion protein from E.coli |
| Amount | 1000 Unit |
| Concentration | ≥1000 Unit/ml |
| Purity | ≥ 95% by SDS-PAGE |
| Form | Liquid |
| Buffer | 20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1 mM TCEP, 50% glycerol |
| Endotoxin level | not determined |
| Application | In vitro research use only |
| Other Features | 1000 Unit of HRV3C Protease is sufficient for cleavage of 100 mg fusion protein. |
| Typical Gel image |  |
| Datasheet & COA | Please contact us to get it. |
Delivery and storage
| Delivery | Cold packs |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Other Notes | Avoid freeze/thaw cycle. Aliquot upon arrival |
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Cleavage of Protein
1. Prepare the target protein (1-2 mg/ml) in cleavage buffer by dialysis. An example of cleavage buffer is 50 mM Tris-HCl, pH 8.0, 150 NaCl, 1 mM DTT.
2. Add HRV3C Protease to the target protein. 1000 units of HRV3C Protease can cleave 100 mg of target protein. Other Protease-to-target protein ratios (w/w) of 1:50 to 1:200 should work for most target proteins.
3. Incubate the cleavage reaction overnight at 4°C for complete cleavage.
