Description

Product Overview

CBI’s recombinant Renilla luciferase (RLuc) protein has 324 amino acids with a molecular weight of 37.4 kDa. It is the full-length protein (aa 1-311) of Renilla luciferase. It also has a 7 × histidine tag on the N-terminus. Thus, anti-His tag antibody can detect the protein in Western blotting. In addition, the recombinant RLuc is expressed by our unique SoluPrx technology and is isolated from the soluble fraction as an active protein. Hence, the recombinant Renilla luciferase (RLuc) Protein is an ideal standard for SDS gels, Western Blots, ELISA, protein array, and function assays.

Product specification

Name	Renilla Luciferase (RLuc), Recombinant
Accession No.	n/a
Target Protein Sequence	n/a
Tag Info	7 x His-tag
Molecular Weight	37.4 kDa
Source	Recombinant protein from E.coli
Amount	100 μg
Concentration	0.5 ~ 1.0 mg/ml
Purity	≥ 95% by SDS-PAGE
Form	Liquid
Buffer	10 mM PBS buffer, 50% glycerol, pH7.2
Endotoxin level	not determined
Application	In vitro research use only
Other Features	n/a
Typical Gel Image
Datasheet & COA	Please contact us to get it.

Delivery and storage

Renilla Luciferase Assay

Renilla luciferase (RLuc) is an oxidative enzyme from Renilla reniformis. It catalyzes the oxidization of renilla luciferin. The RLuc protein provides an extremely bright flash signal that decays rapidly. The protein also has an emission spectrum in the green region. Moreover, the RLuc protein is more stable in serum than the wild-type luciferase. Since its bioluminescence only requires molecular oxygen, Renilla luciferase has been used in numerous applications as a reporter gene in cell culture, in vivo imaging, and other areas.