$0.00
.At Click Biosystems, we offer in vitro transcription (I VT) for custom RNA synthesis. We can generate non-modified and modified RNA transcripts that are multi-kilobase long. Our custom long RNA products are widely used for RNA control templates in real-time PCR (RT-qPCR), external RNA spike-in control assays, in-situ hybridization RNA probes, and mRNA in gene expression.
Synthesis of long RNA oligos by in vitro transcription
Chemical synthesis is a standard method for short RNA oligos, but not for long sequences. In contrast, in vitro transcription (IVT) can generate both short and long RNA transcripts. Hence, we use the in vitro transcription method to generate custom long RNA oligos.
When to choose in vitro transcription?
•Need a long RNA sequence
•Need a messenger RNA (mRNA)
•Need a large amount of RNA
Why choose our custom long RNA synthesis service?
• Careful review of sequences
• All types of long RNA (ssRNA, dsRNA, mRNA, modified, and non-modified)
• Full service from DNA synthesis to RNA production
• Successful delivery of thousands of RNA sequences
• Precise sequence
• High purity by column or PAGE
• Careful handling to avoid cross-contamination
• Removal of DNA template
Features of our custom long RNA synthesis service
• DNA templates
In vitro transcription requires the design and generation of a proper DNA template. We offer full services for custom long RNA oligo synthesis, from DNA to RNA production. We generate custom RNA from verified DNA templates. In addition, to accelerate the project, customers can provide DNA templates (plasmid or PCR product) that are ready for RNA production. The DNA template should have a proper promoter (T7, SP6, or T3) at the 5′-end of the target sequence.
• High purity
We offer PAGE and column purification of RNA products. These methods remove proteins, free rNTPs, small DNA fragments, and salts from the final products.
• No cross-contamination
Trace cross-contamination occurs when multiple RNA samples are processed. Our expert team has strong knowledge and technologies to avoid the risk of cross-contamination between RNA samples.
• Removal of DNA templates
For many applications, the DNA template needs to be removed following RNA production. Our standard protocols degrade DNA templates into smaller fragments and remove them subsequently. We also assess the presence of the trace DNA template in the RNA product. We will report the result to our customer.
• Small to large-scale production
We can synthesize RNA from micrograms to grams.
RNA Examples Made by Click Biosystems Inc.
Please click here to get a quote or email in**@*******os.com.