Beta Galactosidase mRNA (β-gal or LacZ mRNA) expresses galactosidase (β-gal) when transfected into mammalian cells. β-gal catalyzes the hydrolysis of β-galactosides into monosaccharides. In molecular biology, beta-galactosidase is commonly used as a reporter marker. The presence of an active β-gal may be detected by o-nitrophenyl-β-D-galactoside (ONPG), which produces a blue dye when cleaved by the enzyme. The synthetic beta-galactosidase mRNA has a C-terminal c-myc and a His6 tag, which can detect the expression of the enzyme. The mRNA has an m7G cap (Cap-1) and a long poly(A) tail for optimized expression in mammalian systems. It also has a 100% substitution of the modified nucleotide N1-Methylpseudouridine (m1Ψ), which enhances the translation and reduces the innate antiviral response to single-stranded mRNA.
|lyophilized powder from nuclease-free water
|In vitro research use only
Delivery and storage
|Avoid freeze/thaw cycle.
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