Our Cas9 mRNA is a codon-optimized version. This mRNA encodes Cas9 protein with a nuclear localization signal (NLS) at N- and C-termini, which increases the frequency of the delivery of expressed Cas9 protein to the nucleus, resulting in an increased rate of DNA cleavage. In addition, Cas9 mRNA has a C terminal HA epitope sequence that serves as a detection and purification tag.
The synthetic Cas9 mRNA has a cap-1 structure and a long 3′-poly(A) tail. It also has a 100% substitution of the modified nucleotide N1-Methyl-pseudouridine (m1Ψ), which enhances the translation and reduces the host immune response to single-stranded mRNA.
Cas9 mRNA expresses the wild-type Cas9 protein (CRISPR Associated Protein 9) of Streptococcus pyogenes that performs CRISPR/Cas9-mediated genome editing. When co-transfected with a guide RNA (gRNA), the Cas9 protein is directed by the gRNA to cleave the desired gene. Thus, Cas9 mRNA is often used to generate a gene knockout and edit genes by homologous recombination.
|Cas9 mRNA (NLS, HA tag)
|lyophilized powder from nuclease-free water
|In vitro research use only
Delivery and storage
|Avoid freeze/thaw cycle
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