Description
The synthetic firefly luciferase mRNA expresses the firefly luciferase protein when transfected into mammalian cells. The codons of firefly luciferase are optimized for expression in mammalian cells. Firefly luciferase is an oxidative enzyme originally isolated from the firefly (photinus pyralis). It emits bioluminescence in the presence of luciferin and ATP. As a reporter, it is commonly used in mammalian cell culture to measure gene expression and cell viability.
- Codon-optimized for expression in mammalian cells
- U-depletion
- 5′- m7G cap (Cap-1)
- Long polyA120 tail
- 100% substitution of N1-methylpseudouridine
Product specification
| Name | Synthetic firefly luciferase mRNA |
| Amount | 50 μgs |
| Structure | |
| Gel Mobility | Pass |
| Form | lyophilized powder from nuclease-free water |
| Application | In vitro research use only |
Delivery and storage
| Delivery | Cold packs |
| Storage | -80°C |
| Other Notes | Avoid freeze/thaw cycle. |
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