Synthetic Cre Recombinase mRNA (NLS)

Synthetic Cre Recombinase mRNA (NLS)

$225.00

MRNA1016. In stock N/A , .

Description

The synthetic Cre Recombinase mRNA (NLS) expresses the Cre recombinase in transfected mammalian cells. The Cre recombinase gene is fused to a nuclear localization sequence (NLS) that can locate the enzyme to the nucleus where DNA recombination happens. The mRNA has a 5′-Cap-1 structure and a 3’-long poly(A) tail. It also has a 100% substitution of the modified nucleotide N1-methylpseudouridine, which enhances the translation and reduces the innate antiviral response to single-stranded mRNA.

Cre recombinase is a tyrosine recombinase enzyme derived from the P1 bacteriophage. The enzyme uses a topoisomerase I mechanism to carry out site-specific recombination of DNA between loxP sites, which can be located either on the same or on separate pieces of DNA. Microinjection of the synthetic Cre recombinase mRNA induces site-specific recombination of a transgene in mouse oocytes and the success rate of the recombination event is 100%.

Product specification
Name Cre Recombinase mRNA, synthetic
Amount 50 μgs
Structure
Gel Mobility Pass
Form lyophilized powder from nuclease-free water
Application In vitro research use only
Delivery and storage
Delivery Cold packs
Storage -70°C
Other Notes Avoid freeze/thaw cycle.

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