The synthetic Cre Recombinase mRNA (NLS) expresses the Cre recombinase in transfected mammalian cells. The Cre recombinase gene is fused to a nuclear localization sequence (NLS) that can locate the enzyme to the nucleus where DNA recombination happens. The mRNA has a 5′-Cap-1 structure and a 3’-long poly(A) tail. It also has a 100% substitution of the modified nucleotide N1-methylpseudouridine, which enhances the translation and reduces the innate antiviral response to single-stranded mRNA.
Cre recombinase is a tyrosine recombinase enzyme derived from the P1 bacteriophage. The enzyme uses a topoisomerase I mechanism to carry out site-specific recombination of DNA between loxP sites, which can be located either on the same or on separate pieces of DNA. Microinjection of the synthetic Cre recombinase mRNA induces site-specific recombination of a transgene in mouse oocytes and the success rate of the recombination event is 100%.
|Cre Recombinase mRNA, synthetic
|lyophilized powder from nuclease-free water
|In vitro research use only
Delivery and storage
|Avoid freeze/thaw cycle.
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