Description

The synthetic Cre Recombinase mRNA (NLS) expresses the Cre recombinase in transfected mammalian cells. The Cre recombinase gene is fused to a nuclear localization sequence (NLS) that can locate the enzyme to the nucleus where DNA recombination happens. The mRNA has a 5′-Cap-1 structure and a 3’-long poly(A) tail. It also has a 100% substitution of the modified nucleotide N¹-methylpseudouridine, which enhances the translation and reduces the innate antiviral response to single-stranded mRNA.

Cre recombinase is a tyrosine recombinase enzyme derived from the P1 bacteriophage. The enzyme uses a topoisomerase I mechanism to carry out site-specific recombination of DNA between loxP sites, which can be located either on the same or on separate pieces of DNA. Microinjection of the synthetic Cre recombinase mRNA induces site-specific recombination of a transgene in mouse oocytes and the success rate of the recombination event is 100%.

Product specification

Name	Cre Recombinase mRNA, synthetic
Amount	50 μgs
Structure
Gel Mobility	Pass
Form	lyophilized powder from nuclease-free water
Application	In vitro research use only

Delivery and storage

For bulk orders, please click here or email in**@*******os.com.