Description

Product Overview

Our Streptavidin-azide is an azide-modified streptavidin that is designed for site-specific conjugation by click chemistry reaction. The sole azido group is located at the c-terminus of streptavidin. It reacts very efficiently to Dibenzocyclooctyne (DBCO) or alkyne-modified molecules and forms a stable covalent bond. In addition, streptavidin-azide contains a long polyethylene glycol (PEG) spacer (76 atoms) to span the protein and azide. Such a design minimizes the interference between the biotin-binding site and the conjugated molecules. Hence, this innovative streptavidin-azide is extremely useful when random amine-mediated conjugation interferes with the function of the conjugate. In contrast, generally, modification and conjugation of streptavidin is performed on random amines provided by lysine residues and the n-terminal amino acid. Because streptavidin contains at least 5 amino groups, the amine-based conjugation is not site-specific and the products are heterogeneous with a low activity. However, our streptavidin-azide can generate a homogeneous conjugate.

Product specification

Name	Streptavidin-azide
Accession No.	P22629 (amino acids 37-163)
Target Protein Sequence
Tag Info	tag-free
Molecular Weight	14.5 kDa (Monomer) or 58.0 kDa (Tetramer)
Source	Recombinant protein from E.coli
Amount	1.0 mg
Concentration	>1.0 mg/ml
Purity	≥ 95% by SDS-PAGE
Form	Liquid
Buffer	Sodium phosphate buffer, pH7.2
Endotoxin level	not determined
Application	In vitro research use only
Other Features	n/a
Typical Gel Image	batch dependent
Datasheet & COA	Please contact us to get it.

Delivery and storage

Application of azide-modified streptavidin
Antibodies, oligonucleotides, enzymes (HRP, phosphatase), fluorescent dyes (Alexa, ATTO, FITC, R-PE, APC, PerCP), drugs, peptides, and solid supports can be modified by DBCO or alkyne crosslinkers. The modified biomolecules are attached to the c-terminus of Streptavidin-azide at a controllable ratio. In addition, the coupling is very efficient with high yields and purity. Moreover, the conjugates retain the biotin-binding activity.

Streptavidin-azide for site-specific click chemistry conjugation

Application of Streptavidin-azide in bioconjugation

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